> Growth of NIH3T3 cells
> Wound healing assay
> Differentiation into Adipocyte-like cells
Swiss 3T3 L-1 cells imaging for 7 days (1 h-intervals) after replacing to the differentiation-inducing medium
(DMEM, 10 % FBS, 250 nM Dexamethazone, 0.5 mM Isobutylmethylxanthin, 10 ug/mL Insulin)
> IVF bovine embryos
Time-lapse monitoring bovine embryos 6 hours after in vitro fertilization for 9 days (0.5 h-intervals) in an incubator By courtesy of Kei Imai, Department of Sustainable Agriculture, Rakuno Gakuen University, Japan
> GFP fluorescent cell imaging
⇐ Fluorescence and bright field images (using a fluorescent imaging model of CytoWatcher) after transfection of mouse fibroblast NIH3T3 (a 35 mm glass bottom dish) with EGFP expression vector for 48 h (20 minutes-intervals)
(Image processing software ImageJ is used to create merged images and movies)
>Strained tissue section
Masson trichrome stained section preparation of basal ganglia (mammal)
Taken after staining lipid droplets with Oil Red O
4x 8x Digital Zoom 16x Digital Zoom
5mega pixel color CMOS camera. 1.425mm x 1.070mm(2592x1944)
>Usable in CO2 incubator
Since CytoWatcher body is moisture-proof, it can take time-lapsed images of live cells with cultured in a high humidity CO2 incubator. There is no need to use an expensive microscope stage culture system.
As CytoWatcher is compact, it can be used in a limited space
such as a labolatory bench or
a safety cabinet. As it is easy to carry,
you can take it to your work space and use it.
>Just connect to PC with USB
CytoWatcher is used by connecting it to a Windows PC (option) with a USB 3.0 cable
(power is supplied from the PC via USB).
If you start the control software ImageSaverT (included as standard),
you can see live cell images immediately.
You can also save images and take time-lapse images easily.
>Multiple units can be controlled by one PC
Multiple CytoWatchers can be connected to one PC and used
(up to 2 units) for
simultaneous time-lapse imaging of multiple samples in parallel.
The fluorescent imaging model is equipped with a blue LED light source and an excitation / emission filter, and supports green emitted fluorescent imaging such as GFP. It is possible to set it up in a CO2 incubator and combine it with bright field and fluorescent time-lapse imaging.
Purpose and Application
> For bright field cell imaging with low magnification.
> Compact size, no stress to take space.
> Usable in CO2 incubator, for time-lapse imaging in culture condition.
> Equipped with 5M pixels color CMOS camera.
> Multiple samples can be taken by connecting multiple units.
> A model for fluorescent imaging has appeared.
WSL-1800 CytoWatcher is a digital microscope equipped with a 5 megapixel color CMOS camera.
By simply connecting to a Windows PC with a USB cable, you can capture and save microscopic images with a simple operation.
Since CytoWatcher is moisture-proof, it can be placed in a CO2 incubator to take time-lapse images automatically while culturing cells.