Tips for Electrophoresis
Agarose Gel Electrophoresis (Submarine electrophoresis)
What's Agarose Gel Electrophoresis (Submarine Electrophoresis)?
Horizontal agarose gel electrophoresis for nucleic acid (DNA/RNA) samples is operated by the method to immerse gel in a buffer. This method is also called submarine electrophoresis compared to a submarine in the sea. We, ATTO, provide the system of the method called "Submerge Series".
Nucleic acid (DNA/RNA) dissolving in buffer etc. is charged in negative by phosphate residue. If the solution (DNA sample) is added to agarose gel and electrophoresed in buffer, it migrates towards anode (+). At this time, long DNA moves slowly through the network structure on the contrary short DNA moves rapidly due to the molecular sieve effect of the gel. So, nucleic acid shows each mobility depending on the size of nucleic acid. The electrophoresis pattern of nucleic acid is provided by dyeing gel after electrophoresis with a fluorescent dye (Ethidium bromide) and detecting it. You can determine the size (Molecular weight marker is necessary) and get the information of genotype for individual identification from this pattern.
Example of Use： Genotyping
Submerge series is suitable for electrophoresis for analyzing genetic polymorphism because gel size is large and migration distance can be made longer. Also, many samples are operated at once so it is appropriate to electrophoresis for confirming PCR products. The general method for genotyping is the following; DNA extraction from sample-PCR (Polymerase Chain Reaction)-Agarose gel electrophoresis-Gel imaging/analyzing.
How to Select Electrophoresis Chamber
Please select an electrophoresis system depending on the sample. For example, in the case of the experiment with any number of samples (well), we recommend a system in which the width (W) is broad. In the case of an experiment with any number of bands, the system of which length (L) is long is suitable.
How to Select ATTO ECL reagents
Bio-Rad Clarity Western ECL Substrate mid-femto 1705060 200 mL ATTO WSE-7120S (100 mL)
Bio-Rad Clarity Western ECL Substrate mid-femto 1705061 500 mL ATTO WSE-7120L (500 mL)
Millipore Immobilon Western Chemiluminescent HRP Substrate mid-femto WBKLS0100 100 mL ATTO WSE-7120S (100 mL)
Millipore Immobilon Western Chemiluminescent HRP Substrate mid-femto WBKLS0500 500 mL ATTO WSE-7120L (500 mL)
Millipore Immobilon® Classico Western HRP Substrate pico WBLUC0100 100 mL ATTO WSE-7110 (250 mL)
Millipore Immobilon® Classico Western HRP Substrate pico WBLUC0500 500 mL ATTO WSE-7110 (250 mL)
Millipore Immobilon® Crescendo Western HRP Substrate low-pico WBLUR0100 100 mL ATTO WSE-7120S (100 mL)
Millipore Immobilon® Crescendo Western HRP Substrate low-pico WBLUR0500500 mL ATTO WSE-7120L (500 mL)
Millipore Immobilon® Forte Western HRP Substrate mid-femto WBLUF0100 100 mL ATTO WSE-7120S (100 mL)
Millipore Immobilon® Forte Western HRP Substrate mid-femto WBLUF0500 500 mL ATTO WSE-7120L (500 mL)
Thermo Fisher Pierce™ ECL Western Blotting Substrate pico 32209 250 mL ATTO WSE-7110 (250 mL)
Thermo Fisher Pierce™ ECL Western Blotting Substrate pico 3210 950 mL ATTO WSE-7110 (250 mL)
Thermo Fisher Pierce™ ECL Western Blotting Substrate pico 32106 500 mL ATTO WSE-7110 (250 mL)
Thermo Fisher Pierce™ ECL Plus Western Blotting Substrate low-pico 32132100 mL ATTO WSE-7120S (100 mL)
Thermo Fisher Pierce™ ECL Plus Western Blotting Substrate low-pico 32134 25 mL ATTO WSE-7120S (100 mL)
Thermo Fisher Pierce™ ECL Plus Western Blotting Substrate low-pico 32132X3 300 mL ATTO WSE-7120L (500 mL)
Thermo Fisher SuperSignal™ West Pico PLUS Chemiluminescent Substrate low-pico~high femto34577 200 mL ATTO WSE-7120S (100 mL)
Thermo Fisher SuperSignal™ West Pico PLUS Chemiluminescent Substrate low-pico~high femto34580 500 mL ATTO WSE-7120L (500 mL)
Thermo Fisher SuperSignal™ West Pico PLUS Chemiluminescent Substrate low-pico~high femto345781000 mL ATTO WSE-7120L (500 mL)
Thermo Fisher SuperSignal™ West Dura Extended Duration Substrate mid-femto34075 100 mL ATTO WSE-7120S (100 mL)
Thermo Fisher SuperSignal™ West Dura Extended Duration Substrate mid-femto34076 200 mL ATTO WSE-7120L (500 mL)