Tips for Electrophoresis


Agarose Gel Electrophoresis (Submarine electrophoresis)

What's Agarose Gel Electrophoresis (Submarine Electrophoresis)? 

Horizontal agarose gel electrophoresis for nucleic acid (DNA/RNA) sample is operated by the method to immerse gel in buffer. This method is also called submarine electrophoresis compared to a submarine in the sea. We, ATTO, provide the system of method called "Submerge Series".

Nucleic acid (DNA/RNA) dissolving in buffer etc. is charged in negative by phosphate residue. If the solution (DNA sample) is added to agarose gel and electrophoresed in buffer, it migrates towards anode (+). At this time, long DNA moves slowly through network structure on the contrary short DNA moves rapidly due to molecular sieve effect of gel. So, nucleic acid shows each mobility depending on the size of nucleic acid. Electrophoresis pattern of nucleic acid is provided by dyeing gel after electrophoresis with fluorescent dye (Ethidium bromide) and detecting it. You can determine the size (Molecular weight marker is necessary) and get the information of genotype for individual identification from this pattern.

Example of Use : Genotyping 

Submerge series is suitable for electrophoresis for analyzing genetic polymorphism because gel size is large and migration distance can be made longer. Also many number of samples are operated at once so it is appropriate to electrophoresis for confirming PCR products. The general method for genotyping is following; DNA extraction from sample-PCR (Polymerase Chain Reaction)-Agarose gel electrophoresis-Gel imaging/analyzing.

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How to Select Electrophoresis Chamber 

Please select electrophoresis system depending on sample. For example, in the case of the experiment with many number of samples (well), we recommend the system of which width (W) is broad. In the case of experiment with many number of bands, the system of which length (L) is long is suitable.

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How to Select ATTO ECL reagents 

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Bio-Rad                    Clarity Western ECL Substrate mid-femto 1705060 200 mL                                                                      ATTO WSE-7120S (100 mL)

Bio-Rad                    Clarity Western ECL Substrate mid-femto 1705061 500 mL                                                                      ATTO WSE-7120L (500 mL)

Millipore                   Immobilon Western Chemiluminescent HRP Substrate mid-femto WBKLS0100 100 mL                          ATTO WSE-7120S (100 mL)

Millipore                   Immobilon Western Chemiluminescent HRP Substrate mid-femto WBKLS0500 500 mL                          ATTO WSE-7120L (500 mL)

Millipore                   Immobilon® Classico Western HRP Substrate pico WBLUC0100 100 mL                                                ATTO WSE-7110 (250 mL)

Millipore                   Immobilon® Classico Western HRP Substrate pico WBLUC0500 500 mL                                                ATTO WSE-7110 (250 mL)

Millipore                   Immobilon® Crescendo Western HRP Substrate low-pico WBLUR0100 100 mL                                      ATTO WSE-7120S (100 mL)

Millipore                   Immobilon® Crescendo Western HRP Substrate low-pico WBLUR0500500 mL                                       ATTO  WSE-7120L (500 mL)

Millipore                   Immobilon® Forte Western HRP Substrate mid-femto WBLUF0100 100 mL                                             ATTO WSE-7120S (100 mL)

Millipore                   Immobilon® Forte Western HRP Substrate mid-femto WBLUF0500 500 mL                                             ATTO WSE-7120L (500 mL)

Thermo Fisher        Pierce™ ECL Western Blotting Substrate pico 32209 250 mL                                                                     ATTO WSE-7110 (250 mL)

Thermo Fisher        Pierce™ ECL Western Blotting Substrate pico 3210  950 mL                                                                      ATTO WSE-7110 (250 mL)

Thermo Fisher        Pierce™ ECL Western Blotting Substrate pico 32106 500 mL                                                                     ATTO WSE-7110 (250 mL)

Thermo Fisher        Pierce™ ECL Plus Western Blotting Substrate low-pico 32132100 mL                                                       ATTO WSE-7120S (100 mL)

 

Thermo Fisher        Pierce™ ECL Plus Western Blotting Substrate low-pico 32134  25 mL                                                       ATTO WSE-7120S (100 mL)

Thermo Fisher        Pierce™ ECL Plus Western Blotting Substrate low-pico  32132X3 300 mL                                                 ATTO WSE-7120L (500 mL)

Thermo Fisher        SuperSignal™ West Pico PLUS Chemiluminescent Substrate low-pico~high femto34577 200 mL            ATTO WSE-7120S (100 mL)

Thermo Fisher        SuperSignal™ West Pico PLUS Chemiluminescent Substrate low-pico~high femto34580 500 mL            ATTO WSE-7120L (500 mL)

Thermo Fisher        SuperSignal™ West Pico PLUS Chemiluminescent Substrate low-pico~high femto345781000 mL           ATTO WSE-7120L (500 mL)

   

Thermo Fisher        SuperSignal™ West Dura Extended Duration Substrate mid-femto34075 100 mL                                     ATTO WSE-7120S (100 mL)

Thermo Fisher        SuperSignal™ West Dura Extended Duration Substrate mid-femto34076 200 mL                                     ATTO WSE-7120L (500 mL)