U-PAGEL

Precast Gel I Mini Gel 8x9cm

Purpose and Application

Best for High molecular weight proteins separation

Separation and detection of proteins up to 1,500 kDa (UH-T/R310)
SDS-PAGE
Native-PAGE
Screening
Electrophoresis for Western blotting (WB)
DNA (e.g. PCR products) electrophoresis

Features

Suitable for separation of polymer proteins above 500 kDa (UH-T/R5, UH-T/R310)
5~600kDa wide fractional range (UH-T/R420)
Physical strength increases, even low concentration gel is hard to tear
Long-term shelf life 1 year (from manufacturing date)
High speed, short time (30min~) migration possible
Polymer blotting is also good
Wide application (SDS-PAGE, Native-PAGE, DNA)
Tris-Glycine (-SDS) can be used as the migration buffer

Discription

Easy-to-Handle

Polyacrylamide gel is a polymer in which acrylamide and N, N’-Methylenebisacrylamide (Bis), a crosslinking agent, are bonded by radical polymerization. Acrylamide forms a linear structure and the crosslinking agent acts as a bridge to form a polymer (gel) with a net structure. The lower the gel concentration, the larger the pore size, so high molecular protein separation is possible. However, Bis has low solubility, so it becomes a non-uniform gel due to clumps. Non-uniform gels fall in strength and tear easily. Therefore, the problem was that it was difficult to handle.
This time, ATTO succeeded in developing a uniform gel at low concentrations using a new crosslinking agent with higher solubility and longer bonding arms than Bis. It has excellent strength with tensile fracture stress about 1.7 times higher than Bis. In addition, with large pore size, the sample smoothly enters the gel’s net structure without clogging, showing higher separability.