ATTO Products

Precast Gel I Mini Gel 8x9cm

U-PAGEL

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Discription

Purpose and Application

Best for High molecular weight proteins separation

  • Separation and detection of proteins up to 1,500 kDa (UH-T/R310)

  • SDS-PAGE

  • Native-PAGE

  • Screening

  • Electrophoresis for Western blotting (WB)

  • DNA (e.g. PCR products) electrophoresis

 

Features

  • Suitable for separation of polymer proteins above 500 kDa (UH-T/R5, UH-T/R310)

  • 5~600kDa wide fractional range (UH-T/R420)

  • Physical strength increases, even low concentration gel is hard to tear

  • Long-term shelf life 1 year (from manufacturing date)

  • High speed, short time (30min~) migration possible

  • Polymer blotting is also good

  • Wide application (SDS-PAGE, Native-PAGE, DNA)

  • Tris-Glycine (-SDS) can be used as the migration buffer

Easy-to-Handle

Polyacrylamide gel is a polymer in which acrylamide and N, N’-Methylenebisacrylamide (Bis), a crosslinking agent, are bonded by radical polymerization. Acrylamide forms a linear structure and the crosslinking agent acts as a bridge to form a polymer (gel) with a net structure. The lower the gel concentration, the larger the pore size, so high molecular protein separation is possible. However, Bis has low solubility, so it becomes a non-uniform gel due to clumps. Non-uniform gels fall in strength and tear easily. Therefore, the problem was that it was difficult to handle.
This time, ATTO succeeded in developing a uniform gel at low concentrations using a new crosslinking agent with higher solubility and longer bonding arms than Bis. It has excellent strength with tensile fracture stress about 1.7 times higher than Bis. In addition, with large pore size, the sample smoothly enters the gel’s net structure without clogging, showing higher separability.

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Amazing flexibility!!      

Previous Gel production                               

New Gel production                                    

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Pattern data of protein samples

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Gel: UH-T310 (3~10%, 14-well gel)

Sample: WSE-7035 EzStandard HMW, WSE-7020 EzProtein Ladder (MW marker) etc.

Running buffer: AE-1410 EzRun (Tris, Glycine, SDS)

Electrophoresis system:WSE-1165 Mini-Slab

Condition: Constant voltage 300 V, 40 min

Staining: AE-1340 EzStain AQua (CBB staining)

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Gel: UH-T5 (5%, 14-well gel)

Sample: WSE-7035 EzStandard HMW, WSE-7020 EzProtein Ladder (MW marker) etc.

Running buffer: AE-1410 EzRun (Tris, Glycine, SDS)

Electrophoresis system:WSE-1165 Mini-Slab

Condition: Constant voltage 300 V, 40 min

Staining: AE-1340 EzStain AQua (CBB staining)

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Gel: UH-T310 (3~10%, 14-well gel)

Sample: Native PAGE marker etc.

Running buffer: WSE-7055 EzRun TG (Tris, Glycine)

Electrophoresis system:WSE-1165 Mini-Slab

Staining: AE-1340 EzStain AQua (CBB staining)

Gel: UH-T310 (3~10%, 14-well gel)

Sample: DNA ladder etc.

Running buffer: WSE-7055 EzRun TG (Tris, Glycine)

Electrophoresis system:WSE-1165 Mini-Slab

Condition: Constant current 20 mA, 90 min

Staining: WSE-7130 EzFluoroStain DNA

Detection: WSE-6300 LuminoGraph III (Blue LED excitation)

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