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Purpose and Application

  • Extraction of Proteins from Mammalian cell or tissue cultures
  • 2D electrophoresis sample buffer


  • Separate extraction allows higher protein concentration per gel, spot detection is easy.
  • Reducing reagent, alkylating reagent, washing buffer and stratified solutions are also incorporated into a kit
  • Low running cost, while allowing short-time extraction and easy sample preparation
  • Combination use with AE-1430 EzApply (sample preparation kit for SDS-PAGE) makes it applicable to SDS-PAGE as well as 2-D electrophoresis



 AE-1435 EzApply 2D Kit
Major componentsWash buffer: Tris, NaCl
Solution 1: Tris, Ampholine, DTT
Solution 2: Urea, Thiourea, Surfactant
Alkylated reagent: Ampholine, DTT
Stratified solutions: Acrylamide, BPB, Urea
PackageSolution/30 mL bottle x 2 (Wash buffer)
Solution/20 mL bottle (Solution 1)
Solution/10 mL bottle (Solution 2)
Solution/1 mL tube (Alkylated reagent)
Solution/500 μL tube (Stratified solution)
Powder/tube (DTT)
PreparationAppropriated amount of solution is applied to tissues or cells, which were lysed and extracted
Applicable amountExtracted from 100 mg of mammalian tissue 20 runs
Storage6 months at -20℃ (before dissolved in DTT)
1 week after dissolved in DTT

Ordering Information

Code No.DescriptionUnit
2332335AE-1435 EzApply 2D Kit1 pk

AE-1435 EzApply 2D Kit

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