Purpose and Application
Extraction of Protein and PolyPeptide
Sample buffer for SDS-PAGE
Features
Use of dithiothreitol (DTT) as a reducing reagent
Reduction reaction more secure than 2-ME
Less likely to produce false bands than 2-ME
No smell like 2-ME (2-mercaptoethanol)
Low running cost and simple preparation procedure
 
Specifications
 
AE-1430 EzApply
Major components
Tris-HCl buffer, 2% SDS, 20% sucrose, BPB, and 100 mM DTT
(final concentrations: about halves of the above figures)
Package
Solution/30 mL bottle (EzApply solution)
Solution/5 mL bottle x 5 bottles (DTT)
Preparation
EzApply solution is added to DTT bottles and mixed with sample at a ratio of 1:1
Applicable amount
5 mL x 5
Storage
6 months at -20℃ (before dissolved in DTT)
1 week after dissolved in DTT
Ordering Information
Code No.
Description
Unit
2332330
AE-1430 EzApply
1 pk