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Purpose and Application

  • Extraction of Protein and PolyPeptide
  • Sample buffer for SDS-PAGE


  • Use of dithiothreitol (DTT) as a reducing reagent
  • Reduction reaction more secure than 2-ME
  • Less likely to produce false bands than 2-ME
  • No smell like 2-ME (2-mercaptoethanol)
  • Low running cost and simple preparation procedure



 AE-1430 EzApply
Major componentsTris-HCl buffer, 2% SDS, 20% sucrose, BPB, and 100 mM DTT
(final concentrations: about halves of the above figures)
PackageSolution/30 mL bottle (EzApply solution)
Solution/5 mL bottle x 5 bottles (DTT)
PreparationEzApply solution is added to DTT bottles and mixed with sample at a ratio of 1:1
Applicable amount5 mL x 5
Storage6 months at -20℃ (before dissolved in DTT)
1 week after dissolved in DTT

Ordering Information

Code No.DescriptionUnit
2332330AE-1430 EzApply1 pk

AE-1430 EzApply

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