ATTO Products
Chemiluminescence Imaging System
Reference LED Light Source
Centrifuse / Block Incubator / Others
Precast Gel I Compact PAGE Gel 6x6cm
cp-PAGEL
Purpose and Application
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Best for Low molecular weight proteins and polypeptides separation
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Separation and detection of proteins and polypeptides
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SDS-PAGE
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Screening
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Experimental training for students or researchers
Features
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Wide Separation Range of 1,000-75,000Da
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sharp band
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The minimum time for a migration is 15 minutes!Combine fast migration with clean migration patterns
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A year of shelf life
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Stable in quality and reproducibility
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Adopting glass plates with excellent thermostatic efficiency
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Easy to set up and remove gel
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Compatible electrophoresis apparatus: ATTO Compact Gel Size Electrophoresis System (WSE-1010 cPAGE Ace, WSE-1025 cPAGE Ace Twin)
Tips for Low Molecular Weight Protein Electrophoresis
Electrophoresis of proteins and polypeptides with a molecular weight of around 20,000 Da or less is difficult to separate in the usual SDS-PAGE (Laemmli method), so the electrophoresis is carried out with tris-tricin buffer (AE-1415 EzRunT).
Gels and electrophoresis buffers are used exclusively for low molecular weight protein separation. The minimum time for the migration is about 65 minutes, and the migration is completed faster than before.
Low molecular weight proteins and polypeptides can spread if it is not fixed immediately after migration or not stained for a short period of time. CBB staining with AE-1340 EzStain AQua can be stained and destained for a short time using the microwave oven method.
